Glossary of 3B: CAC
Other Decks By This User
- where does CAC take place
- in the mitochondria
- how many carboxylic acids are on citrate?
- 3 - a tricarboxylic acid.
- How does pyruvate get from the cytosol to the mito matrix?
- by an antiporter, pyruvate carrier.
- what happens to pyruvate once it gets into the mito matrix?
- it is oxidatively decarboxylated by pyruvate dehydrogenase complex.
- what links glycolysis and CAC?
- oxidative decarboxylation of pyruvate to give acetyl CoA and CO2
- What does CoA do?
- carries Acyl groups
- What are the components of CoA?
What is the reactive part?
- B-mercaptoethylamine unit
The thiol SH
- how does acetate enter the CAC?
- thioester bonded to CoA.
- what's the diff between ATP and GTP?
- adenine and guanine
- What is Thiamine Pyrophosphate? (TPP)
- The prosthetic group of E1 on pyruvate dehydrogenase complex.
- what is the function of TPP?
- decarboxylate pyruvate
- what is the function of lipoamide?
- what is the function of Coenzyme A in PDHC?
- Acyl carrier
- What is the purpose of FAD in PDHC?
- to reoxidize lipoamide
- what is the function of NAD+ in PDHC?
- to reoxidize FADH2
- how come the reactive part of TPP is reactive?
- The Carbon atom is between N and S, so very stable anion - acidic; deprotonates because pka is about 10.
Then the anion adds to pyruvate to form tetrahedryl intermediate.
- E2 of PDHC
- dihydrolipoyl transacetylase
- What is E3 of PDHC?
- Dihydrolipoyl dehydrogenase
-Oxidizes dihydrolipoamide to regenerate the coenzyme lipoamide.
- what is the reactive center of TPP?
- a thiazolium ring (acidic carbon between a nitrogen and sulfur)
- how many atoms make up the long lipoamide arm of lipoic acid/lysine?
- what are the reactions that take place at E1 of PDHC?
- 1. Decarboxylation - add pyruvate to TPP, give of CO2.
2. Oxidation - oxidize TPP back, transfer Acetyl concomiantly to Lipoamide.
- what are the reactions that take place at E2 of PHDC?
- only one; transfer of acetyl from acetyllipoamide to CoA to leave dihydrolipoamide (reduced form).
- How many enzymes and cofactors are in PDHC
- 3 enzymes
- What are the 5 cofactors in PDHC
- What plays a central role in formation of Acetyl CoA from Pyruvate?
- LIPOIC ACID'S LONG ARM.
- What are the steps of PDHC?
- 1. Thiamine Pyrophosphate decarboxylates
2. Lipoamide Oxidizes the Acetyl group and transfers it to Coenzyme A.
3. FAD reoxidizes Lipoic Acid back to Lipoamide.
4. NAD oxidizes FADH2 back to FAD.
- What is lipoamide linked to on WHICH ENZYME?
- a Lysine residue on Enzyme 2
- What are the cofactors bound to each subunit in TPHC?
- E1: TPP
E2: Lipoamide, CoA
E3: FAD, NAD+
- What is a synthase
- an enzyme catalyzing a synthetic reaction in which 2 units are joined w/out participation of ATP or NTP
- what are control points for the CAC?
You must Login or Register to add cards