Glossary of 1. bone marrow exam and count
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- what are the sites used to obtain bone marrow specimens?
- 1. post/ant iliac crest
3. vertebral spine
- what can you NOT use in bone marrow procedure, why?
- Anticoagulant - makes vacuoles in cells.
- what types of specimens are taken in a bm procedure?
- -aspirate of liquid marrow
- what do you do w/ the aspirate?
- 1. Perform a differential count
2. Calculate the ME ratio
- what is a clot specimen for?
- to evaluate the bm cellularity - normal is 50:50 fat to cells,
hypo = 70:30
hyper = 30:70
- what is the biopsy used for?
- histology processes it, path evaluates it - for bm structure and cellularity
- what special needle is used for a biopsy?
- what five stains are used during bm procedures?
- 1. Romanowsky types
2. Prussian blue - iron evaltn
4. Silver stain
5. Special cytochem. stains
- what is Hematoxylin/eosin for?
- Romanowsky-type stain, to evaluate bm structure and cellularity seen on the biopsy.
- What is the silver stain for?
- to assess the reticulin amounts, which are high in myelofibrosis.
- what 3 things are evaluated during microscopic eval of a biopsy?
- 1. Cellularity
2. Myelofibrosis - reticulin
3. Megakaryocyte quantitation
Same things for clots
- wbat is done with the aspirate specimen?
- a differential - by us!
- where on the smear is a bone marrow aspirate diff done?
- next to a particle, where there
-aren't many RBCs
-are sufficient intact whites
-is an even distribution
- how many cells are counted during the differential?
- 200 to 1000
- what about the megakaryocytes is evaluated?
- -quanity on 10x
-maturation stage (MK1, 2, 3)
- what is the normal ME ratio?
- what are 2 ME ratio abnormalities?
- Myeloid hyperplasia 5:1
Myeloid hypoplasia 1:1 or more
- What is perl's reaction?
what 3 things indicate increased iron stores?
- Evaluation of bone marrow iron stores.
-Normal, ringed, and pathological sideroblasts.
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