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Glossary of micro ch. 3 test 2

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Created by cadomanis

inoculation
introduction of a sample into a container of media to produce a culture of observable growth

isolation
separating one species from another
incubation
conditions that allow growth
inspection
the morphology, number and arrangement of the growth of an organism that has been cultured
identification
the specific genus and species of an organism that was cultured

colony
a mound of cells from which an individual bacterial cell that has been separated from other cells and has space on a nutrient surface that has grown
how many species are in a colony?
one
what are the three types of isolation techniques?
streak plate

pour plate

spread plate



what are the three properties under which media can be classified?
physical state
chemical composition
functional type

what are the different physical states of media?
liquid
semisolid
solid

what are the types of chemical composition that media can be?
synthetic
nonsynthetic
what are the functional types of media?
general purpose
enriched
selective
differential
anaerobic
transport
assay
enumeration






what is liquid media?
a broth that is used that does not solidify
what are semisolids?
media with a clot-like consistency that contains a soildifying agent

can be agar or gelatin

what is solid media?
firm surface for colony formation

contains a solidifying agent and is liquefiable and nonliquifiable

what is the most commonly used solidifying agent?
agar
agar
polysaccharide isolated from red algae

solid at room temp

liquifies at 100 deg. C

provides framework to hold moisture and nutrients

not digetable for most microbes







what are the most commonly used media?
nutrient broth and nutrient agar
nutrient broth
liquid medium containing beef extract and peptone
nutrient agar
solid medium containing beef extract, peptone and agar
synthetic media
contains pure organic and inorganic compounds in an exact chemical formula
complex or nonsynthetic media
contains at least one ingredient that is not chemically definable
general purpose media
media that grows a broad range of microbes, usually nonsynthetic
enriched media
contains complex organic substances such as blood, serum, hemoglobin or special growth factors required by fastidious microbes
selective media
contains one or more agents that inhibits growth of some microbes and encourage growth of the desired microbes
differential media
allows growth of several types of microbes and displays visible differences among desired and undesired microbes
reducing medium
contains a substance that absorbs oxygen or slows penetration of oxygen into medium

used for growing anaerobic bacteria

carbohydrate fermentation medium
contains sugars that can be fermented, converted to acids, and a pH indicator to show the reaction

basis for identifying bacteria and fungi

pure cultures
grows only single known species of microorganisms
mixed cultures
hold two or more identified species or microorganisms
contaminated cultures
once pure or mixed culture that has unwanted microbes growing

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