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Glossary of Strep and Staph differentiation

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Created by steedly

Staphylococcus Aureus

Mannitol

Grows
Ferments
Staphylococcus Aureus

Colonial pigmentation

generally golden yellow
Staphylococcus Aureus

Coagulase

Positive (dark red)
Staphylococcus Aureus

DNase

Positive (red halo)
Staphylococcus Aureus

Hemolysis

Beta
Staphylococcus Aureus

Novobiocin

Sensitive
Staphylococcus Epidermidis

Mannitol Salt Agar

Grows
does not ferment mannitol
Staphylococcus Epidermidis

colonial pigmentation

white
Staphylococcus Epidermidis

Coagulase

Negative
Staphylococcus Epidermidis

DNase

Negative
Staphylococcus Epidermidis

Hemolysis

Negative
Staphylococcus Epidermidis

novobiocin

sensitive
Staphylococcus Saprophyticus

Mannitol Salt Agar

Grows

Does not ferment manntol

Staphylococcus Saprophyticus

colonial pigmentation

white

Staphylococcus Saprophyticus

coagulase

negative
Staphylococcus Saprophyticus

DNase

Negative
Staphylococcus Saprophyticus

Hemolysis

Negative
Staphylococcus Saprophyticus

Novobiocin

resistant
Describe the appearance of mannitol fermenters on MSA.
Yellow Halo
Describe the appearance of coagulase +.
Clot formation (darker red)
Alpha hemolysis
produces a green zone around the colony
Beta hemolysis
clear zone 2-4 times the diameter of the colony
Gamma hemolysis

no hemolysis
Streptococcus Pyogenes

Hemolysis

Beta
Streptococcus Pyogenes

bacitracin

Positive
Enterococcus Faecalis

Hemolysis

alpha --> gamma
Enterococcus Faecalis

Bacitracin

Negative
Streptococcus pneumoniae

hemolysis

Alpha
Streptococcus Pneumoniae

bile solubility

positive

Streptococcus Pneumoniae

optochin

positive
Streptococcus mitis

hemolysis

alpha
Streptococcus mitis

bile solubility

negative

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